Head

Professor Aleksi Sedo, MD, DSc

Researchers

Petr Busek, MD, Ph.D. 
Eva Balaziova, MD, Ph.D. 
Lucie Stollinova Sromova, Ph.D. 
Marek Hilser, MD, Ph.D. 
Zdislava Vanickova, MD
Magdalena Houdova Megova, Ph.D. 

Postgraduate students

Ivana Matrasova, MSc
Lenka Kotackova, MD, MSc
Michal Zubal, MD
Rosana Mateu Sanz, MSc
Petr Vymola, MSc
Nikola Ternerova, MSc
Barbora Vymolova, MD
Tereza Svablova, MD
Elena Garcia Borja, MSc
Dora Konecna, MD

Undergraduate students

Tomas Buna
Jana Veprkova

Technicians

Kvetoslava Vlasicova
Karin Roubickova

Papers and patents of LCCB:

• Šimková A, Bušek P, Šedo A, Konvalinka J.: Molecular recognition of fibroblast activation protein for diagnostic and therapeutic applications. Biochim Biophys Acta Proteins Proteom. 2020 Jul;1868(7):140409. doi: 10.1016/j.bbapap.2020.140409. Epub 2020 Apr 6. PMID: 32171757

• Křepela E., Bušek P., Šedo A.: Nádorové mikroprostředí glioblastomu  (Kapitola 2.), Gliomy, Maxdorf 2018 ISBN:978-80-7345-561-3 

• Škrha J., Frič P., Bušek P., Škrha P., Šedo A..: Sporadic Pancreatic Cancer: Glucose Homeostasis and Pancreatogenic Type 3 Diabetes. Advances in Pancreatic Cancer, InTech 2018 DOI: 10.5772/intechopen.75740

• Sana J, Busek P, Fadrus P, Besse A, Radova L, Vecera M, Reguli S, Stollinova Sromova L, Hilser M, Lipina R, Lakomy R, Kren L, Smrcka M, Sedo A, Slaby O.: Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival. Sci Rep. 2018 Feb 12;8(1):2836. doi: 10.1038/s41598-018-20929-6.   one of the top 100 read cell and molecular biology papers for Scientific Reports in 2018  

• Bušek P, Mateu R, Zubal M, Kotačková L, Šedo A.: Targeting fibroblast activation protein in cancer - Prospects and caveats.Front Biosci (Landmark Ed). 2018 Jun 1;23:1933-1968.
• Jobe NP, Živicová V, Mifková A, Rösel D, Dvořánková B, Kodet O, Strnad H, Kolář M, Šedo A, Smetana K Jr, Strnadová K, Brábek J, Lacina L.: Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes. Histochem Cell Biol. 2018 May;149(5):503-516. doi: 10.1007/s00418-018-1650-4.
• Dvořáková P, Bušek P, Knedlík T, Schimer J, Etrych T, Kostka L, Stollinová Šromová L, Šubr V, Šácha P, Šedo A, Konvalinka J: Inhibitor-Decorated Polymer Conjugates Targeting Fibroblast Activation Protein. J Med Chem. 2017 Oct 26;60(20):8385-8393. doi: 10.1021/acs.jmedchem.7b00767 
• Krepela E, Busek P, Hilser M, Vanickova Z, Sedo A.: Species-specific real-time RT-PCR analysis of expression of stromal cell genes in a tumor xenotransplantation model in mice. Biochem Biophys Res Commun. 2017 Sep 9;491(1):126-133. doi: 10.1016/j.bbrc.2017.07.061 
  
• Frič P, Šedo A, Škrha J, Bušek P, Laclav M, Škrha P, Zavoral M.: Early detection of sporadic pancreatic cancer: time for change. Eur J Gastroenterol Hepatol. 2017 Aug;29(8):885-891. doi: 10.1097/MEG.0000000000000904
• Matrasova I, Busek P, Balaziova E, Sedo A.: Heterogeneity of molecular forms of dipeptidyl peptidase-IV and fibroblast activation protein in human glioblastomas Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017 Sep;161(3):252-260. doi: 10.5507/bp.2017.010
  
• Frič P, Škrha J, Šedo A, Bušek P, Laclav M, Bunganič B, Zavoral M.: Precursors of pancreatic cancer, Eur J Gastroenterol Hepatol. 2017 Mar;29(3):e13-e18 doi: 10.1097/MEG.0000000000000810
• Škrha J, Bušek P, Uhrová J, Hrabal P, Kmochová K, Laclav M, Bunganič B, Frič P.:  Lower plasma levels of glucose-dependent insulinotropic peptide (GIP) and pancreatic polypeptide (PP) in patients with ductal adenocarcinoma of the pancreas and their relation to the presence of impaired glucoregulation and weight loss. Pancreatology. 2017 Jan - Feb;17(1):89-94, doi: 10.1016/j.pan.2016.12.004
• Dvořánková B, Szabo P, Kodet O, Strnad H, Kolář M, Lacina L, Krejčí E, Naňka O, Šedo A, Smetana K Jr.: Intercellular crosstalk in human malignant melanoma. Protoplasma. 2017 May;254(3):1143-1150. doi: 10.1007/s00709-016-1038-z
• Smetana K Jr, Lacina L, Szabo P, Dvořánková B, Brož P, Šedo A.: Ageing as an Important Risk Factor for Cancer. Anticancer Res. 2016 Oct;36(10):5009-5017
  
• Fric P, Skrha J, Sedo A, Zima T, Busek P, Kmochova K, Laclav M, Bunganic B, Solar S, Hrabal P, Belina F, Zaruba P, Skrha P, Zavoral M. : Early detection of pancreatic cancer: impact of high-resolution imaging methods and biomarkers. Eur J Gastroenterol Hepatol. 2016 Dec;28(12):e33-e43.
• Busek P, Balaziova E, Matrasova I, Hilser M, Tomas R, Syrucek M, Zemanova Z, Krepela E, Belacek J, Sedo A. : Fibroblast activation protein alpha is expressed by transformed and stromal cells and is associated with mesenchymal features in glioblastoma. Tumour Biol. 2016 Oct;37(10):13961-13971.
• Sromova L, Busek P, Posova H, Potockova J, Skrha P, Andel M, Sedo A.: The effect of dipeptidyl peptidase-IV inhibition on circulating T cell subpopulations in patients with type 2 diabetes mellitus. Diabetes Res Clin Pract. 2016 Aug;118:183-92. doi: 10.1016/j.diabres.2016.06.020.
• Busek P, Vanickova Z, Hrabal P, Brabec M, Fric P, Zavoral M, Skrha J, Kmochova K, Laclav M, Bunganic B, Augustyns K, Van Der Veken P, Sedo A. : Increased tissue and circulating levels of dipeptidyl peptidase-IV enzymatic activity in patients with pancreatic ductal adenocarcinoma. Pancreatology. 2016 Sep-Oct;16(5):829-38. doi: 10.1016/j.pan.2016.06.001.
• Fric P, Skrha J, Sedo A, Busek P, Kmochova K, Laclav M, Solar S, Bunganic B, Zavoral M.: Early pancreatic carcinogenesis - risk factors, early symptoms, and the impact of antidiabetic drugs. Eur J Gastroenterol Hepatol. 2016 Jul;28(7):e19-25. doi: 10.1097/MEG.0000000000000646.
• Fric P, Sedo A, Skrha J, Zima T, Busek P, Skrha P, Kmochova K, Laclav M, Bunganic B, Zavoral M. Early detection of sporadic pancreatic cancer. Cas Lek Cesk. 2016;155(1):44-7
• Sromova L, Busek P, Sedova L, Sedo A.: Intraindividual changes of dipeptidyl peptidase-IV in peripheral blood of patients with rheumatoid arthritis are associated with the disease activity. BMC Musculoskelet Disord. 2015 Sep 9;16:244
• Trylcova J, Busek P, Smetana K Jr, Balaziova E, Dvorankova B, Mifkova A, Sedo A.: Effect of cancer-associated fibroblasts on the migration of glioma cells in vitro. Tumour Biol. 2015 Aug;36(8):5873-9.
• Sedo A., Mentlein R.: Glioma cell biology, Springer 2014 product flyer
  The book Glioma cell biology received two awards from the commitee of the Czech Oncological Society of the Czech Medical Association of J.E. Purkyně. link 1 link 2
• Busek P, Hrabal P., Fric P., Sedo A.: Co-expression of the homologous proteases fibroblast activation protein and dipeptidyl peptidase-IV in the adult human Langerhans islets. Histochem Cell Biol. 2015 May;143(5):497-504
• Lakomy R., Slampa P., Trebicky F., Fardus. P., Benes V., Smrcka M., Kolar Z., Hermanova M., Sedo A., Belanova R., Slaby O. : Doporučené postupy Neuroonkologické sekce České onkologické společnosti ČLS JEP , Ambit Media, 2014. link
  
• Busek P.,Sedo A.: Dipeptidyl Peptidase-IV and Related Proteases in Brain Tumors (Chapter 8.), Evolution of the Molecular Biology of Brain Tumors and the Therapeutic Implications, InTech 2013.
• Busek P, Stremenova J, Sromova L, Hilser M, Balaziova E, Kosek D, Trylcova J, Strnad H, Krepela E, Sedo A.: Dipeptidyl peptidase-IV inhibits glioma cell growth independent of its enzymatic activity. Int J Biochem Cell Biol. 44(5):738-47, 2012.

  The article received an award from the commitee of the Czech Oncological Society of the Czech Medical Association of J.E. Purkyně.

• Mares V., Stremenova J, Lisa V., Kozakova H., Marek J., Syrucek M., Soula O., Sedo A. : Compartment & malignance-dependent up-regulation of γ-glutamyl transpeptidase and dipeptidyl peptidase-IV activity in human brain gliomas. Histol Histopathol 27(7):931-40, 2012.
• Hilser M., Trylcova J., Busek P., Sedo A. Limitations of Macroscopic Fluorescence Imaging for the Estimation of Tumor Growth in an Orthotopic Glioma Mouse Model, Folia Biol 58(3):128-33 2012.
• Balaziova E., Busek P., Stremenova J., Sromova L., Krepela E., Lizcova L., Sedo A.: Coupled expression of dipeptidyl peptidase-IV and fibroblast activation protein-α in transformed astrocytic cells. Mol Cell Biochem 354(1-2): 283-9, 2011.
• Sromova L., Mareckova H., Sedova L., Balaziova E., Sedo A.: Dipeptidyl peptidase-IV in synovial fluid and in synovial fluid mononuclear cells of patients with rheumatoid arthritis. Clin Chim Acta. 411(15-16): 1003-1164, 2010.
• Stulc T., Sedo A.: Inhibition of multifunctional dipeptidyl peptidase-IV: Is there a risk of oncological and immunological adverse effects? Diabetes Res Clin Pract. 88(2): 125-3, 2010
• Stremenova J., Mares V., Lisa V., Hilser M., Krepela E., Vanickova Z., Syrucek M., Soula O., Sedo A.: Expression of dipeptidyl peptidase-IV activity and/or structure homologs in human meningiomas. Int J Oncol. 36(2): 351-8, 2010.
• Stremenova J., Mares V., Lisa V., Hilser M., Krepela E., Vanickova Z., Syrucek M., Soula O., Sedo A.: Expression of dipeptidyl peptidase-IV activity and/or structure homologs in human meningiomas. Int J Oncol. 36(2): 351-8, 2010.
• Sedo A., Stremenova J., Busek P., Duke-Cohan J.: Dipeptidyl peptidase-IV and related molecules: markers of malignancy? Expert Opinion on Medical Diagnostics. 2(6): 677-689, 2008.
• Busek P., Stremenova J., Krepela E., Sedo A.: Modulation of substance P signaling by dipeptidyl peptidase-IV enzymatic activity in human glioma cell lines Physiol Res. 57(3): 443-9, 2008.
• Busek P., Stremenová J., Sedo A.: Dipeptidyl peptidase-IV enzymatic activity bearing molecules in human brain tumors - good or evil? Front Biosci. 13: 2319-26, 2008.
• Stremenova J., Krepela E., Mares V., Trim J., Dbaly V., Marek J., Vanickova Z., Lisa V., Yea C, Sedo A.: Expression and enzymatic activity of dipeptidyl peptidase-IV in human astrocytic tumours are associated with tumour grade. Int J Oncol. 31(4): 785-92, 2007.
• Sedo A., Balaziova E., Sedova L., Duke-Cohan J.: Dipeptidyl peptidase-IV activity and/or structure homologues (DASH): Contributing factors in the pathogenesis of rheumatoid arthritis? Arthritis Res Ther. 7(6): 253-269, 2005.
• Mares V., Malik R., Lisa V., Sedo A.: Up-regulation of gamma-glutamyltransferase (GGT) activity in growth modulated astrocytic C6 glial cells in culture. Mol Brain Res. 136(1-2): 75-80, 2005.
• Krajci D., Mares V., Lisa V., Bottone MG., Pellicciari C.: Intranuclear microtubules are hallmarks of an unusual form of cell death in cisplatin-treated C6 glioma cells. Histochem Cell Biol. 125(1-2): 183-91, 2006.
• Balaziova E., Sedova L., Mares V., Vlasicova K., Sevcik J., Sedo A.: Dipeptidyl Peptidase-IV Activity and/or Structure Homologs (DASH): Contributing Factors in the Pathogenesis of Rheumatic Diseases? Adv Exp Med Biol. 575: 169-74, 2006.
• Busek P., Krepela E., Mares V., Vlasicova K., Sevcik J., Sedo A.: Expression and Function of Dipeptidyl Peptidase IV and Related Enzymes in Cancer. Adv Exp Med Biol. 575: 55-62, 2006.
• Sedo A., Busek P., Scholzova E., Malik R., Vlasicova K., Janackova S., Mares V.: Dipeptidyl peptidase-IV activity and/or structure homologues“ (DASH) in growth modulated glioma cell lines. Biol Chem. 385(6): 557-559, 2004.
• Busek P., Malik R., Sedo A.: Dipeptidyl peptidase IV activity and/or structure homologues (DASH) and their substrates in cancer. Int J Biochem Cell Biol. 36(3): 408-421, 2004.
• Mares V., Lisa V., Malik R., Kozakova H., Sedo A.: Cisplatin induced gamma-glutamyltransferase up-regulation, hypertrophy and differentiation in astrocytic glioma cells in culture. Histol. Histopathol. 18(3): 687-693, 2003.
• Sedo A., Malik R.: Dipeptidyl peptidase IV-like molecules: homologous proteins or homologous activities? Biochim Biophys Acta. 1550(2): 107-16, 2001.